| Primary Identifier | MGI:6448061 | Allele Type | Endonuclease-mediated |
| Attribute String | Recombinase | Gene | Uts2b |
| Strain of Origin | FVB/N | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 genome editing is used to insert a T2A-Cre cassette immediately upstream of the endogenous stop codon, a 1kb upstream homology arm, and a 2kb downstream homology arm with a TG to GT mutation introduced into the 3' UTR (114-115bp after stop codon) to avoid cleavage by the sgRNA. The T2A oligopeptide mediates ribosomal skipping and is derived from foot-and-mouth disease virus 2A from the insect Thosea asigna virus. T2A permits cre recombinase to be expressed in frame with Uts2b as a separate polypeptide. |
