| First Author | Ovnic M | Year | 1991 |
| Journal | Genomics | Volume | 9 |
| Issue | 2 | Pages | 344-54 |
| PubMed ID | 1840565 | Mgi Jnum | J:11055 |
| Mgi Id | MGI:59497 | Doi | 10.1016/0888-7543(91)90263-e |
| Citation | Ovnic M, et al. (1991) Characterization of a murine cDNA encoding a member of the carboxylesterase multigene family. Genomics 9(2):344-54 |
| abstractText | We have characterized a mRNA sequence containing the entire coding region of a mouse carboxylesterase (EC 3.1.1.1). pEs-N, an 1840-bp composite of five overlapping cDNA clones, contains an open reading frame of 554 amino acids that display a high degree of similarity with rat and rabbit carboxylesterases. Genetic mapping studies place this carboxylesterase in cluster 1 of the esterase region on chromosome 8. Results of blot hybridization analysis of genomic DNA probed with a pEs-N cDNA under both low and high stringency conditions suggest membership in a carboxylesterase multigene family, as would be expected for a nonspecific carboxylesterase. A message size of 1850-1900 nucleotides was revealed by RNA blot hybridization analysis. S1 nuclease protection analyses with a probe representing a segment of pEs-N detected message in liver, kidney, and lung, but not in spleen, brain, testes, and submandibular gland, with higher levels in female than in male kidney. Additional S1 nuclease-protected mRNA species were found, suggesting the expression of distinct members of a multigene family. In vitro translation of a full-size transcript of pEs-N resulted in a product of 51.5 kDa. Upon the addition of microsomes, this product was processed into a protein of 60.4 kDa, which is within the size range of monomeric units of mouse carboxylesterases. |
