| First Author | Pöpperl H | Year | 1992 |
| Journal | EMBO J | Volume | 11 |
| Issue | 10 | Pages | 3673-80 |
| PubMed ID | 1356763 | Mgi Jnum | J:2381 |
| Mgi Id | MGI:50904 | Doi | 10.1002/j.1460-2075.1992.tb05452.x |
| Citation | Popperl H, et al. (1992) An autoregulatory element of the murine Hox-4.2 gene. EMBO J 11(10):3673-80 |
| abstractText | Hox-4.2 promoter activity was assayed by transient expression assays in P19 embryonal carcinoma (EC) cells. Cotransfection of a luciferase reporter gene construct driven by Hox-4.2 upstream sequences with an expression vector for the Hox-4.2 gene product resulted in a 20-fold increase in luciferase activity. This activity was specific in that the Hox-1.6 gene product had no effect in the same assay. Mutational analysis defined a cis-acting element with enhancer function which conferred most of this increase. Activation was largely dependent on two TAAT/ATTA motifs within this 217 bp fragment and HOX-4.2 bound specifically to both of these motifs. The 217 bp element maps within a highly conserved region of the human Hox-4.2 gene (HOX4B) which has been shown to display spatial enhancer activity in mice and flies. These findings suggest a conserved autoregulatory mechanism for the control of Hox-4.2 expression. |
