| First Author | Cerretti DP | Year | 1999 |
| Journal | Cytokine | Volume | 11 |
| Issue | 8 | Pages | 541-51 |
| PubMed ID | 10433800 | Mgi Jnum | J:57143 |
| Mgi Id | MGI:1343898 | Doi | 10.1006/cyto.1998.0466 |
| Citation | Cerretti DP, et al. (1999) Characterization of the cDNA and gene for mouse tumour necrosis factor alpha converting enzyme (TACE/ADAM17) and its location to mouse chromosome 12 and human chromosome 2p25. Cytokine 11(8):541-51 |
| abstractText | Numerous proteins are cleaved or ''shed'' from their membrane-bound form. One such protein, tumour necrosis factor alpha (TNF-alpha), is synthesized as a type 2 transmembrane protein. Recently, a human protease responsible for this shedding, the TNF-alpha converting enzyme (TACE/ADAM17), was isolated. TACE/ADAM17 is a member of the adamalysin class of zinc-binding metalloproteases or ADAM (a disintegrin and metalloprotease). We report the isolation and characterization of the mouse TACE/ADAM17 cDNA and gene, Mouse TACE/ADAM17 has a 92% amino-acid identity with the human protein and was ubiquitously expressed. A recombinant form of the protease is found to cleave a peptide representing the cleavage site of precursor mouse TNF-alpha.An alternatively spliced form of mouse TACE/ ADAM17 was found that would produce a soluble protein. The gene for TACE/ADAM17 is approximately 50 kb and contains 19 exons, Chromosomal mapping places TACE/ADAM17 on mouse chromosome 12 and human chromosome 2p25. (C) 1999 Academic Press. |
