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Interaction Term : systematic evolution of ligands by exponential enrichment [MI:0657] PSI Molecular Interactions

Description  RNA and cDNA constructs with variable central sequences and a constant flanking region are collected in a complex library. The library is then screened to select either specific binding partners of a bait molecule (generally a protein) or particular enzymatic activities of the nucleic acid molecules themselves. The selected nucleic acids are amplified using the constant flanking regions to increase their abundance. Cycles of selection-amplification can be repeated to increase the specificity of the targets that, at the end, are individually identified by sequencing. Namespace  PSI-MI
Obsolete  false
  • synonyms:
  • in vitro evolution of nucleic acids,
  • selex
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Ontology

Ontology Term --> All ancestors

Ontology Term --> All descendants

Ontology Term --> Direct children

Ontology Term --> Direct parents

 

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