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Interaction Term : bimolecular luminiscence complementation [MI:2170] PSI Molecular Interactions

Description  The bimolecular luminescence complementation (BiLC) is an assay for determination of protein interactions and/or their location in living cells. This approach is based on complementation between two fragments of a bioluminescent enzyme such as luciferin or aequorin. Interactions between proteins fused to each fragment bring the fragments together resulting in the reconstitution of a fully functional bioluminescent enzyme that can be identified through microscopy or luminescence detection. Namespace  PSI-MI
Obsolete  false
  • synonyms:
  • luminescence complementation assay,
  • split-luciferase complementation assay,
  • bioluminescence complementation assay,
  • split-luciferase complementation,
  • bioluminescence complementation,
  • bilc,
  • luminiscence complementation
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