| First Author | Fotia AB | Year | 2006 |
| Journal | Int J Biochem Cell Biol | Volume | 38 |
| Issue | 3 | Pages | 472-9 |
| PubMed ID | 16337426 | Mgi Jnum | J:387762 |
| Mgi Id | MGI:8354415 | Doi | 10.1016/j.biocel.2005.11.006 |
| Citation | Fotia AB, et al. (2006) The ubiquitin-protein ligases Nedd4 and Nedd4-2 show similar ubiquitin-conjugating enzyme specificities. Int J Biochem Cell Biol 38(3):472-9 |
| abstractText | Nedd4 and Nedd4-2 are closely related HECT-type ubiquitin-protein ligases (E3) implicated in the regulation of a number of proteins and pathways. Given the close homology between these E3 enzymes it would be predicted that a conserved ubiquitin-conjugating enzyme (E2) specificity exists between the two proteins. However, E2 specificities for Nedd4 and Nedd4-2 are not well established. In the present studies we aimed at clarifying the E2-specificities of Nedd4 and Nedd4-2 using in vitro ubiquitination assays. We demonstrate strong substrate ubiquitination in the presence of UbcH5b by both Nedd4 and Nedd4-2. We also found that Ube2e3, an E2 previously shown to be used by Nedd4-2, is used less efficiently than UbcH5b. Our results suggest that for optimal ubiquitination Nedd4 and Nedd4-2 require the same E2 enzymes. |
