| First Author | Hilgers J | Year | 1976 |
| Journal | Mouse News Lett | Volume | 54 |
| Pages | 30-1 | Mgi Jnum | J:13688 |
| Mgi Id | MGI:61870 | Citation | Hilgers J (1976) Inbred strains and markers. Mouse News Lett 54:30-1 |
| abstractText | Full text of MNL contribution: Inbred strains and markers. Forty conventional inbred mouse strains are presently being bred in the Netherlands Cancer Institute. The official designation of these strains is given in the table, together with the following 11 genetic marker patterns (see also MNL 48, p. 19 for additional isoenzyme patterns of some of these strains as given by Nichols, Ruddle and Muhlbock): 1. Ahh (Taylor, Life Sci. 10, 1127, 1971). The allele for aryl hydrocarbon hydroxylase has been determined with the technique of application of DMBA on the skin as described by Taylor. Note that the exceptional strains, that is the strains which do not develop lesions in a 10 day period, are the two AKR strains (the AKR/FuRdA is the high leukemia strain of the two) and the two DBA substrains, DBA/HeA and DBA/LiA, as well as one strain of European origin DD/He/fA. It should be noted that although the WLL/BrA and WLL/Br/eBA strains are scored as positive, they develop minimal lesions over the 10 day observation period, perhaps indicative of a variant allele of the Ahh locus. Tested by Dr. J. Hilkens. 2. Hbb (Popp, Fed. Proc. 24, 347, 1965; Petras and Martin, Bioch. Gen. 3, 303, 1969). Note that all strains on C57BL background, including e.g. BIMA/A, BIR/A and P/A strains have the s (=single) genotype. A surprise was the finding of the Hbb-s allele in two closely related strains of European origin, the GRS/A and MAS/A strains (for origin of these strains as well as LIS, LTS and STS strains, see Staats, Cancer Res. 32, 1609, 1972). This marker was determined by Dr. H. Hoffman (NCI, Bethesda, USA), Dr. F. Ruddle and Miss E. Nichols (Yale Un., New Haven, USA) and by Dr. J. Hilkens (Amsterdam). 3. Hc (Cinader et al., J. Exp. Med. 120, 897, 1964). The results on the distribution of the MuB1 antigen or Hc (hemolytic complement) component correspond very well with those reported in the original paper of Cinader, e.g. on the striking difference between the DBA/He and DBA/Li strains. Since the DBA/LiA came from Little in 1930 to Amsterdam, which was before the split-up of the DBA/1 and DBA/2 substrains, the genotype with the Hc factor seems the original one for this strain. Note that the majority of the European strains, especially of the group which were brought to Amsterdam from Switzerland (Grundig, Zurich in 1955) by Muhlbock, lack the MuB1 antigen. The antiserum to determine the presence of this antigen in the serum of mice was kindly provided by Dr. Cinader from Toronto; the immunodiffusion tests were carried out by Dr. J. Hilgers. 4. Trf (Shreffler, Proc. Nat. Acad. Sci. USA, 46, 1378, 1960). The Trf or transferring marker distinguishes the CBA/BrA strain from all other strains. Tests were carried out by Drs. A. Groen and J. Hilkens in the Veterinary Faculty of the University of Utrecht. 5. Es-5 (Petras and Biddle, Can. J. Genet. Cytol. 9, 704, 1967). The DD/He/fA strain was the only strain with the Es-5a allele. The marker was also determined by Drs. Groen and Hilkens in Utrecht (see under 4). (Prof. Dr. J. Bouw and Dr. L. van Zutphen). 6-9. Ly-1, Ly-2, Ly-3 and Ly-5 (Itakura et al., Transpl., 13, 239, 1972; Komuro et al., Immunogenetics, 1, 452, 1975). The patterns for the lymphocyte antigens closely follow expected group differences on the basis of a large variety of isoenzyme and other genetic markers published previously for these strains. Note the similarity of the A, BALB/c and C57BL pattern: bbba. Note also that the C3H-DBA series of strains has its own pattern: aaba. Note furthermore that the rare Ly-5b marker links the LIS/A, LTS/A and STS/A strains and distinguishes them from the GRS/A-MAS/A group. This combination of three European strains has also a unique esterase (Dr. Hoffman, personal communication) as well as a rare Eg-1 (egasin) genotype (Dr. Paigen, Roswell Park, Buffalo, USA), two markers on chromosome 8. The Ly patterns were obtained by Dr. F.W. Shen in Dr. E. Boyse's Department of Sloan-Kettering. 10. Thy.1 (Reif and Allen, J. Exp. Med. 120, 413, 1964). This pattern was given previously (MNL 50, p. 25) and it serves here to show the uniqueness of the high leukemia AKR/FuRdA strain. It is as such comparable with the rare Ly-3a allele present in this strain only. 11. rd (Sidman and Green, J. Hered. 56, 23, 1965). This recessive marker leading to blindness was not only present in the C3H/HeA and C3H/He/fa strains of American origin, but also the STS/A and TSI/A strains of European origin. This marker was determined by Dr. R. van Nie. Researchers who want to obtain additional information about other genetic markers of those 40 strains (a, b, c, d, Es-1, Es-3, Mod-1, Id-1, Gpd-1, Gpi-1, Pgm-1, Ldr-1, Dip-1, Mup-1, Mpi, Svp, If-1, cdm, Fv-1, Fv-2, Ig-1, Tla, Ss, Mod-2, 6-Gpd, Es-7, Pgk, Es-2, Gpd-2, Pep-B, Pep-S, Np, Gpo, Ada, Ldh-A, Ldh-B, Mor-1, Mor-2, Got-1, Got-2 and Pgm-2) may write to Dr. J. Hilgers. For information about H-2 write to Dr. A. Dux. Strain: A/BrA; Marker: Ahh: +; Hbb: d; Hc: o; Trf: b; Es-5: b; Ly-1: b; Ly-2: b; Ly-3: b; Ly-5: a; Thy.1: b; rd: +. Strain: A/Br/fA; Marker: Ahh: +; Hbb: d; Hc: o; Trf: b; Es-5: b; Ly-1: b; Ly-2: b; Ly-3: b; Ly-5: a; rd: +. Strain: A/Sna; Marker: Hbb: d; Hc: o; Trf: b; Es-5: b. Strain: A2G/GA; Marker: Ahh: +; Hbb: d; Hc: o; Trf: b; Es-5: b; Ly-1: b; Ly-2: b; Ly-3: b; Ly-5: a; Thy.1: b; rd: +. Strain: AKR/FuA; Marker: Hbb: d; Hc: o; Trf: b; Es-5: b; Thy.1: b. Strain: AKR/FuRdA; Marker: Hbb: d; Hc: o; Trf: b; Es-5: b; Ly-1: b; Ly-2: a; Ly-3: a; Ly-5: a; Thy.1: a; rd: +. Strain: BALB/c/Crg1A; Marker: Ahh: +; Hbb: d; Hc: 1; Trf: b; Es-5: b; Ly-1: b; Ly-2: b; Ly-3: b; Ly-5: a; Thy.1: b; rd: +. Strain: BALB/c/HeA; Marker: Ahh: +; Hbb: d; Hc: 1; Trf: b; Es-5: b; Ly-1: b; Ly-2: b; Ly-3: b; Ly-5: a; Thy.1: b; rd: +. Strain: BALB/cHefC3H/HeA; Marker: Ahh: +; Hbb: d; Hc: 1; Trf: b; Es-5: b; Ly-1: b; Ly-2: b; Ly-3: b; Ly-5: a. Strain: BALB/c/ByA; Marker: Ahh: +; Hbb: d; Hc: 1; Trf: b; Es-5: b; Ly-1: b; Ly-2: b; Ly-3: b; Ly-5: a. Strain: BIMA/A; Marker: Ahh: +; Hbb: s; Hc: 1; Trf: b; Es-5: b; Ly-1: b; Ly-2: b; Ly-3: b; Ly-5: a; Thy.1: b; rd: +. Strain: BIR/A; Marker: Ahh: +; Hbb: s; Hc: 1; Trf: b; Es-5: b; Ly-1: b; Ly-2: b; Ly-3: b; Ly-5: a; Thy.1: b. Strain: C57BL/LiA; Marker: Ahh: +; Hbb: s; Hc: 1; Trf: b; Es-5: b; Ly-1: b; Ly-2: b; Ly-3: b; Ly-5: a; Thy.1: b; rd: +. Strain: C57BL/6ByA; Marker: Ahh: +; Hbb: s; Hc: 1; Trf: b; Es-5: b; Ly-1: b; Ly-2: b; Ly-3: b; Ly-5: a; Thy.1: b; rd: +. Strain: C57BL/10ScSnA; Marker: Ahh: +; Hbb: s; Hc: 1; Trf: b; Es-5: b; Ly-1: b; Ly-2: b; Ly-3: b; Ly-5: a; Thy.1: b. Strain: C57BL/MHeA; Marker: Ahh: +; Hbb: s; Hc: 1; Trf: b; Es-5: b; Ly-1: b; Ly-2: b; Ly-3: b; Ly-5: a; Thy.1: b. Strain: CBA/BrA; Marker: Ahh: +; Hbb: d; Hc: 1; Trf: a; Es-5: b; Ly-1: a; Ly-2: a; Ly-3: b; Ly-5: a; Thy.1: b; rd: +. Strain: C3H/HeA; Marker: Hbb: d; Hc: 1; Trf: b; Es-5: b; Ly-1: a; Ly-2: a; Ly-3: b; Ly-5: a; Thy.1: b; rd: rd. Strain: C3H/He/fA; Marker: Hbb: d; Hc: 1; Trf: b; Es-5: b; Ly-1: a; Ly-2: a; Ly-3: b; Ly-5: a; rd: rd. Strain: C3H/SnHeDiA; Marker: Hbb: d; Hc: 1; Trf: b; Es-5: b; Ly-1: a; Ly-2: a; Ly-3: b; Ly-5: a. Strain: DBA/HeA; Marker: Ahh: +; Hbb: d; Hc: o; Trf: b; Es-5: b; Thy.1: b; rd: +. Strain: DBA/LiA; Marker: Ahh: -; Hbb: d; Hc: 1; Trf: b; Es-5: b; Ly-1: a; Ly-2: a; Ly-3: b; Ly-5: a; Thy.1: b; rd: +. Strain: DBA/Li/fA; Marker: Ahh: -; Hbb: d; Hc: 1; Trf: b; Es-5: b; Ly-1: a; Ly-2: a; Ly-3: b; Ly-5: a; Thy.1: a; rd: +. Strain: DD/He/fA; Marker: Ahh: -; Hbb: d; Hc: o; Trf: b; Es-5: a; Ly-1: b; Ly-2: b; Ly-3: b; Ly-5: b; Thy.1: b; rd: +. Strain: GRS/A; Marker: Ahh: +; Hbb: s; Hc: o; Trf: b; Es-5: b; Ly-1: b; Ly-2: a; Ly-3: b; Ly-5: a; Thy.1: b; rd: +. Strain: IF/BrA; Marker: Hbb: d; Hc: 1; Trf: b; Es-5: b; Ly-1: b; Ly-2: a; Ly-3: b; Ly-5: a. Strain: LIS/A; Marker: Ahh: +; Hbb: d; Hc: o; Trf: b; Es-5: b; Ly-1: b; Ly-2: b; Ly-3: b; Ly-5: b; Thy.1: b. Strain: LTS/A; Marker: Ahh: +; Hbb: d; Hc: o; Trf: b; Es-5: b; Ly-1: b; Ly-2: b; Ly-3: b; Ly-5: b; Thy.1: b; rd: +. Strain: LTS/fA; Marker: Ahh: +; Hbb: d; Hc: o; Trf: b; Es-5: b; Ly-1: b; Ly-2: b; Ly-3: b; Ly-5: b; rd: +. Strain: MAS/A; Marker: Ahh: +; Hbb: s; Hc: o; Trf: b; Es-5: b; Ly-1: b; Ly-2: a; Ly-3: b; Ly-5: a; Thy.1: b; rd: +. Strain: 020/A; Marker: Ahh: +; Hbb: d; Hc: o; Trf: b; Es-5: b; Ly-1: b; Ly-2: a; Ly-3: b; Ly-5: a; Thy.1: b; rd: +. Strain: OIR/A; Marker: Ahh: +; Hbb: d; Hc: o; Trf: b; Es-5: b; Ly-1: b; Ly-2: a; Ly-3: b; Ly-5: a; Thy.1: b; rd: +. Strain: P/A; Marker: Ahh: +; Hbb: s; Hc: 1; Trf: b; Es-5: b; Thy.1: b; rd: +. Strain: RIII/SeA; Marker: Ahh: +; Hbb: d; Hc: 1; Trf: b; Es-5: b; Ly-1: b; Ly-2: b; Ly-3: b; Ly-5: b; rd: +. Strain: RIII/Se/fA; Marker: Hbb: d; Hc: 1; Trf: b; Es-5: b; Ly-1: b; Ly-2: b; Ly-3: b; Ly-5: b; rd: +. Strain: STS/A; Marker: Ahh: +; Hbb: d; Hc: o; Trf: b; Es-5: b; Ly-1: b; Ly-2: b; Ly-3: b; Ly-5: b; Thy.1: b; rd: rd. Strain: TSI/A; Marker: Hbb: d; Hc: 1; Trf: b; Es-5: b; Ly-1: b; Ly-2: b; Ly-3: b; Ly-5: a; Thy.1: b; rd: rd. Strain: WLL/BrA; Marker: Ahh: +; Hbb: d; Hc: 1; Trf: b; Es-5: b; Ly-2: b; Ly-3: b; Ly-5: a; Thy.1: b. Strain: WLL/Br/eBA; Marker: Ahh: +; Hbb: d; Hc: 1; Trf: b; Es-5: b; Ly-1: a; Ly-2: b; Ly-3: b; Ly-5: a. Strain: 129/JA; Marker: Hbb: d. |
