| First Author | Gotoh M | Year | 2004 |
| Journal | FEBS Lett | Volume | 562 |
| Issue | 1-3 | Pages | 134-40 |
| PubMed ID | 15044014 | Mgi Jnum | J:114109 |
| Mgi Id | MGI:3688345 | Doi | 10.1016/S0014-5793(04)00219-4 |
| Citation | Gotoh M, et al. (2004) Molecular cloning and characterization of beta1,4-N-acetylgalactosaminyltransferases IV synthesizing N,N'-diacetyllactosediamine. FEBS Lett 562(1-3):134-40 |
| abstractText | A sequence highly homologous to beta1,4-N-acetylgalactosaminyltransferase III (beta4GalNAc-T3) was found in a database of human expressed sequence tags. The full-length open reading frame of the gene, beta4GalNAc-T4 (GenBank accession number AB089939), was cloned using the 5' rapid amplification of cDNA ends method. It encodes a typical type II transmembrane protein of 1039 amino acids having 42.6% identity with beta4GalNAc-T3. The recombinant enzyme transferred N-acetylgalactosamine to N-acetylglucosamine-beta-benzyl with a beta1,4-linkage to form N,N'-diacetyllactosediamine as did beta4GalNAc-T3. In specificity toward oligosaccharide acceptor substrates, it was quite similar to beta4GalNAc-T3 in vitro, however, the tissue distributions of the two enzymes were quite different. These results indicated that the two enzymes have similar roles in different tissues. |
