| First Author | Kwon BS | Year | 1989 |
| Journal | Biochem Biophys Res Commun | Volume | 158 |
| Issue | 1 | Pages | 1-10 |
| PubMed ID | 2783549 | Mgi Jnum | J:9554 |
| Mgi Id | MGI:58014 | Doi | 10.1016/s0006-291x(89)80168-8 |
| Citation | Kwon BS, et al. (1989) The structure of the mouse lymphocyte pore-forming protein perforin. Biochem Biophys Res Commun 158(1):1-10 |
| abstractText | Purified murine lymphocyte pore-forming protein (PFP or perforin) was partially sequenced. Oligonucleotides synthesized on the basis of this sequence information were used to screen a murine cytotoxic T lymphocyte (CTL) cDNA library. Seven clones were obtained, two of which were sequenced, providing full-length sequence information on PFP. Murine PFP (534 a.a.) is 68% identical to human PFP. Hydropathic analysis revealed a predominantly hydrophilic protein with some hydrophobic domains, including a region (a.a. 191-251) that could contain putative membrane-spanning domains. PFP is approx. 20% identical to human C7, C8 and C9 within a region encompassing 270 a.a., confirming previous immunological cross-reactivity studies. Northern blot analysis showed that expression of PFP but not of a serine esterase transcript is enhanced in a CTL line by antigen receptor-stimulation. Southern blot analysis of mouse genomic DNA indicated that PFP is encoded as a single-copy gene with the coding region contained within 10 kilobases of genomic DNA. |
