| First Author | Kouhara H | Year | 1991 |
| Journal | Biochem Biophys Res Commun | Volume | 176 |
| Issue | 1 | Pages | 31-7 |
| PubMed ID | 1708247 | Mgi Jnum | J:26802 |
| Mgi Id | MGI:74233 | Doi | 10.1016/0006-291x(91)90885-b |
| Citation | Kouhara H, et al. (1991) Expression cDNA cloning of fibroblast growth factor (FGF) receptor in mouse breast cancer cells: a variant form in FGF-responsive transformed cells. Biochem Biophys Res Commun 176(1):31-7 |
| abstractText | Expression cDNA library of fibroblast growth factor (FGF)-responsive mouse breast cancer cells (SC-3) was prepared and screened using chick FGF receptor (FGF-R) cDNA as a probe. Two positive clones were isolated. Sequence analysis revealed that two clones have an identical full open reading frame. Compared with sequence data on mouse brain FGF-R, SC-3 cells were found to contain FGF-R with 12 amino acids insertion near N-terminal region. This insertion was mainly composed of hydrophobic amino acids. Additionally, the deletion of two amino acids in extracellular domain and the substitution of one amino acid in C-terminal region were identified in SC-3 cell FGF-R. Transfection of this clone into CHO cells resulted in a significant increase in basic FGF (bFGF) binding. |
