First Author | Chui DH | Year | 1995 |
Journal | Biochem Biophys Res Commun | Volume | 209 |
Issue | 1 | Pages | 40-6 |
PubMed ID | 7726861 | Mgi Jnum | J:24214 |
Mgi Id | MGI:71963 | Doi | 10.1006/bbrc.1995.1467 |
Citation | Chui DH, et al. (1995) cDNA cloning of murine Nrf 2 gene, coding for a p45 NF-E2 related transcription factor. Biochem Biophys Res Commun 209(1):40-6 |
abstractText | A lambda gt11 cDNA library was constructed from poly-A rich RNA prepared from circulating murine yolk sac derived nucleated erythroblasts, on day 9 of gestation. In addition to the embryonic globin genes, this library was found to contain clones for the hematopoietic specific p45 NF-E2, as well as p18 NF-E2 and p45 NF-E2 related factor 1 (Nrf 1) genes. Using a degenerate oligonucleotide 17mer probe coding for a part of the highly conserved DNA binding domain for p45 NF-E2, we have isolated murine Nrf 2, a second murine homologue related to p45 NF-E2. The murine Nrf 2 gene is expressed not only in erythroid cells, but also in the 3T3 murine fibroblast cell line. |