| First Author | Burbelo PD | Year | 1998 |
| Journal | Biochim Biophys Acta | Volume | 1443 |
| Issue | 1-2 | Pages | 203-10 |
| PubMed ID | 9838117 | Mgi Jnum | J:51802 |
| Mgi Id | MGI:1326948 | Doi | 10.1016/s0167-4781(98)00207-3 |
| Citation | Burbelo PD, et al. (1998) Cloning, genomic organization and chromosomal assignment of the mouse p190-B gene. Biochim Biophys Acta 1443(1-2):203-10 |
| abstractText | The p190 family of GTPases consists of at least two different isoforms both containing an N-terminal GTPase and a C-terminal Rho GAP domain. Here we have isolated and characterized genomic and cDNA clones spanning the entire coding region of the mouse p190-B gene. Genomic data were obtained by sequencing plasmid subclones of two overlapping mouse genomic phage clones. Interestingly, a single 3.9 kb exon was found to contain approx. 80% of the coding region of the mouse p190-B protein (amino acid residues 1-1238) including the 5'-untranslated region, the N-terminal GTPase domain and a middle domain of unknown function. Missing from this exon, however, was the C- terminal Rho GAP domain, which was cloned from mouse brain mRNA using reverse transcriptase polymerase chain reaction. Comparison of the mouse with the human p190-B proteins revealed that approx. 97% of the amino acid residues were identical. Northern analysis of total RNA from a variety of mouse tissues detected ubiquitous expression of two p190-B transcripts of 4.0 and 6.8 kb in size. Analysis of two multilocus genetic crosses localized the mouse gene, Gfi2, to a position on chromosome 12, consistent with the mapping of the human gene to a position of conserved synteny on chromosome 14. The high level of sequence homology between the human and the mouse suggests that there is a strong selective pressure to maintain the p190-B protein structure. (C) 1998 Elsevier Science B.V. All rights reserved. |
