| First Author | Delpire E | Year | 1994 |
| Journal | J Biol Chem | Volume | 269 |
| Issue | 41 | Pages | 25677-83 |
| PubMed ID | 7929272 | Mgi Jnum | J:21042 |
| Mgi Id | MGI:69092 | Doi | 10.1016/s0021-9258(18)47302-4 |
| Citation | Delpire E, et al. (1994) Molecular cloning and chromosome localization of a putative basolateral Na(+)-K(+)-2Cl- cotransporter from mouse inner medullary collecting duct (mIMCD-3) cells. J Biol Chem 269(41):25677-83 |
| abstractText | Electroneutral Na(+)-K(+)-2Cl- cotransporters represent one of the major routes for Cl- movement in epithelia. A secretory form of the cotransporter has been described in the basolateral membrane of a variety of epithelia from fish to mammals. We isolated a putative bumetanide-sensitive Na(+)-K(+)-2Cl- cotransporter cDNA, BSC2, from mIMCD-3 cells. Northern analysis indicates that in contrast to BSC1, the recently cloned renal-specific apical isoform of the cotransporter, BSC2 is expressed in secretory epithelia and thus appears to represent the basolateral isoform. Furthermore, BSC2 is also expressed in non-polarized cells, such as red cells and myocytes. Sequence comparison and chromosome localization demonstrate that BSC2 and BSC1 are different genes that diverged before the evolution of vertebrates. |
