| First Author | Matsuo M | Year | 1996 |
| Journal | Biochim Biophys Acta | Volume | 1309 |
| Issue | 1-2 | Pages | 21-4 |
| PubMed ID | 8950170 | Mgi Jnum | J:36774 |
| Mgi Id | MGI:84197 | Doi | 10.1016/s0167-4781(96)00123-6 |
| Citation | Matsuo M, et al. (1996) Cloning and expression of the cDNA for mouse NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase. Biochim Biophys Acta 1309(1-2):21-4 |
| abstractText | The cDNA for mouse NAD+ dependent 15-hydroxyprostaglandin dehydrogenase (15-PGDH) was isolated from a lung cDNA library. The cDNA contains a 798 bp open reading frame that codes for a protein of 266 amino acids (M(r) 28775) which shares 87% identity with the human 15-PGDH protein. The regions that are believed to form the NAD+ binding site and the active site are conserved in the mouse and human enzymes. The authenticity of the mouse cDNA was confirmed by expression of an active 15-PGDH in Escherichia coli. Northern blot analysis demonstrated that 15-PGDH mRNA is expressed primarily in lung, intestine, stomach and liver. |
