| First Author | van de Bospoort R | Year | 2012 |
| Journal | J Cell Biol | Volume | 199 |
| Issue | 6 | Pages | 883-91 |
| PubMed ID | 23229896 | Mgi Jnum | J:195236 |
| Mgi Id | MGI:5476894 | Doi | 10.1083/jcb.201208024 |
| Citation | van de Bospoort R, et al. (2012) Munc13 controls the location and efficiency of dense-core vesicle release in neurons. J Cell Biol 199(6):883-91 |
| abstractText | Neuronal dense-core vesicles (DCVs) contain diverse cargo crucial for brain development and function, but the mechanisms that control their release are largely unknown. We quantified activity-dependent DCV release in hippocampal neurons at single vesicle resolution. DCVs fused preferentially at synaptic terminals. DCVs also fused at extrasynaptic sites but only after prolonged stimulation. In munc13-1/2-null mutant neurons, synaptic DCV release was reduced but not abolished, and synaptic preference was lost. The remaining fusion required prolonged stimulation, similar to extrasynaptic fusion in wild-type neurons. Conversely, Munc13-1 overexpression (M13OE) promoted extrasynaptic DCV release, also without prolonged stimulation. Thus, Munc13-1/2 facilitate DCV fusion but, unlike for synaptic vesicles, are not essential for DCV release, and M13OE is sufficient to produce efficient DCV release extrasynaptically. |
