| First Author | Kopito RR | Year | 1989 |
| Journal | Cell | Volume | 59 |
| Issue | 5 | Pages | 927-37 |
| PubMed ID | 2686841 | Mgi Jnum | J:16498 |
| Mgi Id | MGI:64573 | Doi | 10.1016/0092-8674(89)90615-6 |
| Citation | Kopito RR, et al. (1989) Regulation of intracellular pH by a neuronal homolog of the erythrocyte anion exchanger. Cell 59(5):927-37 |
| abstractText | We have isolated AE3, a novel gene expressed primarily in brain neurons and in heart. The predicted AE3 polypeptide shares a high degree of identity with the anion exchange and cytoskeletal binding domains of the erythrocyte band 3 protein. Expression of AE3 cDNA in COS cells leads to chronic cytoplasmic acidification and to chloride- and bicarbonate-dependent changes in intracellular pH, confirming that this gene product is an anion exchanger. Characterization of an AE3 mutant lacking the NH2-terminal 645 amino acids demonstrates that the COOH-terminal half of the polypeptide is both necessary and sufficient for correct insertion into the plasma membrane and for anion exchange activity. The NH2-terminal domain may play a role in regulating the activity of the exchanger and may be involved in the structural organization of the cytoskeleton in neurons. |
