| First Author | Sun W | Year | 2001 |
| Journal | Biochem Biophys Res Commun | Volume | 280 |
| Issue | 4 | Pages | 1048-54 |
| PubMed ID | 11162633 | Mgi Jnum | J:74709 |
| Mgi Id | MGI:2158993 | Doi | 10.1006/bbrc.2000.4244 |
| Citation | Sun W, et al. (2001) PAL31, a nuclear protein required for progression to the S phase. Biochem Biophys Res Commun 280(4):1048-54 |
| abstractText | PAL31 is a nuclear protein expressed by various cell types. In the present study, the expression and function of PAL31 were examined in the cytokine-regulated growth of T and B cell lines. Treatment of the cells with mitogens [ovine PRL, recombinant rat placental lactogen-I (PL-I) and human IL-3] caused a dose-dependent increase in the expression of PAL31 mRNA in the PRL-dependent cell line Nb2, and IL-3 dependent cell line BaF3. A time-course study on synchronized Nb2 cells revealed that the expression of PAL31 is specific to the late G1 and S phases. Immunocytological studies revealed that PAL31 accumulates in the nuclei at the S phase. Furthermore, the antisense oligonucleotide for PAL31 severely inhibited the proliferation of Nb2 cells by inhibiting cells progressing to the S phase. Thus, PAL31 is a nuclear protein associated with cell cycle progression. |
