| First Author | Daniel F | Year | 1983 |
| Journal | EMBO J | Volume | 2 |
| Issue | 7 | Pages | 1061-5 |
| PubMed ID | 6354707 | Mgi Jnum | J:7212 |
| Mgi Id | MGI:55683 | Doi | 10.1002/j.1460-2075.1983.tb01546.x |
| Citation | Daniel F, et al. (1983) Structure and expression of the mouse beta 2-microglobulin gene isolated from somatic and non-expressing teratocarcinoma cells. EMBO J 2(7):1061-5 |
| abstractText | Mouse teratocarcinoma cells express neither H-2 heavy chains nor beta 2-microglobulin (beta 2-m). We have constructed two genomic libraries, one from PCC4-aza-RI embryonal carcinoma cells and the other from their adult syngenic counterpart 129/Sv liver cells (H-2bc). The libraries were screened with a full length mouse beta 2-m cDNA probe which we isolated and sequenced. Two cosmid clones carrying the entire beta 2-m gene were isolated, one from each library. There was no detectable difference in structure between the two genes. Furthermore, both were shown to be active and to restore beta 2-m synthesis upon transfer into mutant cells deficient in beta 2-m. Irreversible DNA alterations in or around the beta 2-m gene are thus unlikely to account for the lack of beta 2-m gene expression in embryonal teratocarcinoma cells. |
