| First Author | Schieldrop PJ | Year | 1996 |
| Journal | Biochim Biophys Acta | Volume | 1308 |
| Issue | 2 | Pages | 111-3 |
| PubMed ID | 8764827 | Mgi Jnum | J:34800 |
| Mgi Id | MGI:82268 | Doi | 10.1016/0167-4781(96)00103-0 |
| Citation | Schieldrop PJ, et al. (1996) Isolation of a murine glucose-dependent insulinotropic polypeptide (GIP) cDNA from a tumor cell line (STC6-14) and quantification of glucose-induced increases in GIP mRNA. Biochim Biophys Acta 1308(2):111-3 |
| abstractText | A 537 base pair cDNA clone for murine GIP has been isolated. The elucidated sequence encodes an open reading frame of 432 base pairs which codes for a 144 amino acid precursor. Murine GIP is predicted to differ from the human hormone by three amino acid substitutions: arginine for histidine at position 18, arginine for lysine at position 30 and serine for lysine at position 34. GIP mRNA levels in STC6-14 cells incubated in the presence of varying glucose concentrations was investigated using a competitive-PCR method. In the presence of a 5-mM glucose stimulus, 1 x 10(5) GIP cells were found to contain 3.9 +/- 0.59 amol of GIP mRNA while the same number of cells contained 11.6 +/- 1.4 amol when subjected to a high (25 mM) glucose stimulus. |
