| First Author | Combadiere C | Year | 1998 |
| Journal | Biochem Biophys Res Commun | Volume | 253 |
| Issue | 3 | Pages | 728-32 |
| PubMed ID | 9918795 | Mgi Jnum | J:51793 |
| Mgi Id | MGI:1326935 | Doi | 10.1006/bbrc.1998.9849 |
| Citation | Combadiere C, et al. (1998) Gene cloning, RNA distribution, and functional expression of mCX3CR1, a mouse chemotactic receptor for the CX3C chemokine fractalkine. Biochem Biophys Res Commun 253(3):728-32 |
| abstractText | Human fractalkine and its apparent murine counterpart neurotactin are the only members identified so far of the CX3C subfamily of chemokines. Recently, a human fractalkine receptor was identified and named CX3CR1. Here we have identified a mouse counterpart of this receptor. The receptor was identified by analysis of a mouse genomic clone named PC2 isolated by homology hybridization using CX3CR1 as probe. Clone PC2 has a 354-codon open reading frame that has 83% amino acid identity to CX3CR1. PC2 RNA was abundant in brain and lung and comparatively less abundant in lung, liver, kidney, testis, and peripheral blood leukocytes, a pattern similar to that found for CX3CR1. The recombinant fractalkine, but no other chemokines tested, induced chemotaxis and transient increases in [Ca2+]i in HEK 293 cells transfected with PC2, whereas untransfected cells did not respond. Furthermore, fractalkine bound specifically to the transfected cells (Kd=4 nM). Thus, fractalkine is a functional ligand for this receptor and we propose to name it mCX3CR1 for murine CX3C chemokine receptor 1. |
