| First Author | Bal NC | Year | 2012 |
| Journal | Nat Med | Volume | 18 |
| Issue | 10 | Pages | 1575-9 |
| PubMed ID | 22961106 | Mgi Jnum | J:193427 |
| Mgi Id | MGI:5468397 | Doi | 10.1038/nm.2897 |
| Citation | Bal NC, et al. (2012) Sarcolipin is a newly identified regulator of muscle-based thermogenesis in mammals. Nat Med 18(10):1575-9 |
| abstractText | The role of skeletal muscle in nonshivering thermogenesis (NST) is not well understood. Here we show that sarcolipin (Sln), a newly identified regulator of the sarco/endoplasmic reticulum Ca(2+)-ATPase (Serca) pump, is necessary for muscle-based thermogenesis. When challenged to acute cold (4 degrees C), Sln(-/-) mice were not able to maintain their core body temperature (37 degrees C) and developed hypothermia. Surgical ablation of brown adipose tissue and functional knockdown of Ucp1 allowed us to highlight the role of muscle in NST. Overexpression of Sln in the Sln-null background fully restored muscle-based thermogenesis, suggesting that Sln is the basis for Serca-mediated heat production. We show that ryanodine receptor 1 (Ryr1)-mediated Ca(2+) leak is an important mechanism for Serca-activated heat generation. Here we present data to suggest that Sln can continue to interact with Serca in the presence of Ca(2+), which can promote uncoupling of the Serca pump and cause futile cycling. We further show that loss of Sln predisposes mice to diet-induced obesity, which suggests that Sln-mediated NST is recruited during metabolic overload. These data collectively suggest that SLN is an important mediator of muscle thermogenesis and whole-body energy metabolism. |
