| First Author | Flamant S | Year | 2003 |
| Journal | Mamm Genome | Volume | 14 |
| Issue | 1 | Pages | 21-30 |
| PubMed ID | 12532265 | Mgi Jnum | J:81437 |
| Mgi Id | MGI:2449350 | Doi | 10.1007/s00335-002-3032-3 |
| Citation | Flamant S, et al. (2003) Characterization of a putative type IV aminophospholipid transporter P-type ATPase. Mamm Genome 14(1):21-30 |
| abstractText | The P-type ATPases comprise a well-studied family of proteins involved in the active transport of charged substrates across biological membranes. Starting from a mouse bone marrow-derived macrophage cDNA library and using a signal peptide trapping strategy, we identified a new P-type ATPase family member. We characterized the genomic structure of this gene, named Atp10d, as well as its human counterpart. The presence of P-type ATPase consensus motifs and phylogenetic analysis showed that this gene is a member of the type IV, putative amphipath transporters subfamily. We showed that this gene is expressed in kidney and placenta. We also found that the C57BL/6 strain carries a constitutive stop codon in the sequence of Atp10d exon 12, whereas 14 other inbred mouse strains show an uninterrupted reading frame at this location. This mutation in C57BL/6 should lead to a non-functional protein, suggesting that this gene may not be essential. We discuss the involvement of the Atp10d gene in the fat-prone phenotype of the C57BL/6 strain and its physical mapping within a QTL associated with HDL-cholesterol levels. |
