| First Author | Glozak MA | Year | 2003 |
| Journal | Mol Endocrinol | Volume | 17 |
| Issue | 1 | Pages | 27-41 |
| PubMed ID | 12511604 | Mgi Jnum | J:73215 |
| Mgi Id | MGI:2154719 | Doi | 10.1210/me.2002-0192 |
| Citation | Glozak MA, et al. (2003) Trapping and characterization of novel retinoid response elements. Mol Endocrinol 17(1):27-41 |
| abstractText | Retinoids, such as retinoic acid (RA), play a critical role in normal vertebrate development and physiology. However, embryonic exposure to excess retinoids also causes severe malformations. Retinoids bind RA receptors and retinoid X receptors, thus activating a plethora of genes. Separating the genes induced directly by retinoid-bound receptors from those induced subsequently by other transcription factors is difficult. The loose consensus defining known RA responsive elements (RAREs) further complicates this effort. We developed a yeast-based system to trap functional RAREs in the mouse genome. Several of the clones contain RAREs near RA-induced genes. Mammalian reporter gene analyses and EMSAs showed that these are bona fide RAREs. This functional genomics approach should identify RA-regulated genes that initiate critical signaling cascades in cells. |
