| First Author | Moulds BA | Year | 1994 |
| Journal | Mutat Res | Volume | 311 |
| Issue | 1 | Pages | 1-7 |
| PubMed ID | 7526162 | Mgi Jnum | J:29989 |
| Mgi Id | MGI:77511 | Doi | 10.1016/0027-5107(94)90067-1 |
| Citation | Moulds BA, et al. (1994) Spontaneous mutation at codon 61 of the Ha-ras gene in the nascent liver of B6C3F1, C3H/He and C57BL/6 mice. Mutat Res 311(1):1-7 |
| abstractText | DNA was isolated from the liver of young B6C3F1, C3H/He and C57BL/6 mice, 6-9 weeks old. A portion of exon 2 of Ha-ras was amplified by PCR allele-specific amplification. The PCR product was identified by (a) size, (b) presence of a diagnostic restriction site, and (c) direct sequencing. Our results indicate that nascent mouse liver bears a subpopulation of cells which contain a mutation in codon 61 of Ha-ras, specifically an A to G transition at position 2. Therefore, the detection of this mutation in chemically induced mouse liver tumors does not demonstrate that the chemical in question acts as a mutagen. It might act by a nongenotoxic mechanism, i.e., by facilitating a clonal expression of cells bearing this spontaneous mutation. |
