| First Author | Shayeghi M | Year | 2005 |
| Journal | Cell | Volume | 122 |
| Issue | 5 | Pages | 789-801 |
| PubMed ID | 16143108 | Mgi Jnum | J:122693 |
| Mgi Id | MGI:3715073 | Doi | 10.1016/j.cell.2005.06.025 |
| Citation | Shayeghi M, et al. (2005) Identification of an intestinal heme transporter. Cell 122(5):789-801 |
| abstractText | Dietary heme iron is an important nutritional source of iron in carnivores and omnivores that is more readily absorbed than non-heme iron derived from vegetables and grain. Most heme is absorbed in the proximal intestine, with absorptive capacity decreasing distally. We utilized a subtractive hybridization approach to isolate a heme transporter from duodenum by taking advantage of the intestinal gradient for heme absorption. Here we show a membrane protein named HCP 1 (heme carrier protein 1), with homology to bacterial metal-tetracycline transporters, mediates heme uptake by cells in a temperature-dependent and saturable manner. HCP 1 mRNA was highly expressed in duodenum and regulated by hypoxia. HCP 1 protein was iron regulated and localized to the brush-border membrane of duodenal enterocytes in iron deficiency. Our data indicate that HCP 1 is the long-sought intestinal heme transporter. |
