| First Author | Seale P | Year | 2000 |
| Journal | Cell | Volume | 102 |
| Issue | 6 | Pages | 777-86 |
| PubMed ID | 11030621 | Mgi Jnum | J:64793 |
| Mgi Id | MGI:1889987 | Doi | 10.1016/s0092-8674(00)00066-0 |
| Citation | Seale P, et al. (2000) Pax7 is required for the specification of myogenic satellite cells. Cell 102(6):777-86 |
| abstractText | The paired box transcription factor Pax7 was isolated by representational difference analysis as a gene specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization revealed that Pax7 was also expressed in satellite cells residing in adult muscle. Cell culture and electron microscopic analysis revealed a complete absence of satellite cells in Pax7(-/-) skeletal muscle. Surprisingly, fluorescence-activated cell sorting analysis indicated that the proportion of muscle-derived stem cells was unaffected. Importantly, stem cells from Pax7(-/-) muscle displayed almost a 10-fold increase in their ability to form hematopoietic colonies. These results demonstrate that satellite cells and muscle-derived stem cells represent distinct cell populations. Together these studies suggest that induction of Pax7 in muscle-derived stem cells induces satellite cell specification by restricting alternate developmental programs. |
