| First Author | Brock VJ | Year | 2022 |
| Journal | Sci Adv | Volume | 8 |
| Issue | 5 | Pages | eabl9770 |
| PubMed ID | 35119925 | Mgi Jnum | J:325893 |
| Mgi Id | MGI:6876595 | Doi | 10.1126/sciadv.abl9770 |
| Citation | Brock VJ, et al. (2022) P2X4 and P2X7 are essential players in basal T cell activity and Ca(2+) signaling milliseconds after T cell activation. Sci Adv 8(5):eabl9770 |
| abstractText | Initial T cell activation is triggered by the formation of highly dynamic, spatiotemporally restricted Ca(2+) microdomains. Purinergic signaling is known to be involved in Ca(2+) influx in T cells at later stages compared to the initial microdomain formation. Using a high-resolution Ca(2+) live-cell imaging system, we show that the two purinergic cation channels P2X4 and P2X7 not only are involved in the global Ca(2+) signals but also promote initial Ca(2+) microdomains tens of milliseconds after T cell stimulation. These Ca(2+) microdomains were significantly decreased in T cells from P2rx4(-/-) and P2rx7(-/-) mice or by pharmacological inhibition or blocking. Furthermore, we show a pannexin-1-dependent activation of P2X4 in the absence of T cell receptor/CD3 stimulation. Subsequently, upon T cell receptor/CD3 stimulation, ATP release is increased and autocrine activation of both P2X4 and P2X7 then amplifies initial Ca(2+) microdomains already in the first second of T cell activation. |
