| First Author | Polvino WJ | Year | 1992 |
| Journal | Somat Cell Mol Genet | Volume | 18 |
| Issue | 5 | Pages | 443-50 |
| PubMed ID | 1475710 | Mgi Jnum | J:3670 |
| Mgi Id | MGI:52179 | Doi | 10.1007/BF01233084 |
| Citation | Polvino WJ, et al. (1992) Molecular cloning and nucleotide sequence of cDNA encoding a functional murine low-density-lipoprotein receptor. Somat Cell Mol Genet 18(5):443-50 |
| abstractText | The low-density-lipoprotein (LDL) receptor is an important mediator of mammalian cholesterol metabolism; its congenital absence in humans is characterized by hypercholesterolemia, atherosclerosis, and coronary artery disease. We report here the identification and cloning of a cDNA encoding the murine LDL receptor. The cDNA insert is 4467 base pairs in length and the deduced amino acid sequence bears 78.2% homology with the reported human sequence. This murine cDNA was subcloned into a retroviral-based expression vector, LmLSN1, and transfected into 3T3 cells. The production of functional LDL receptor was confirmed by ligand binding of DiI-LDL cholesterol. |
