| First Author | Cai JP | Year | 2003 |
| Journal | Biochem Biophys Res Commun | Volume | 305 |
| Issue | 4 | Pages | 1073-7 |
| PubMed ID | 12767940 | Mgi Jnum | J:83864 |
| Mgi Id | MGI:2663996 | Doi | 10.1016/s0006-291x(03)00864-7 |
| Citation | Cai JP, et al. (2003) Mouse MTH2 protein which prevents mutations caused by 8-oxoguanine nucleotides. Biochem Biophys Res Commun 305(4):1073-7 |
| abstractText | MutT-related proteins degrade 8-oxo-7,8-dihydrodeoxyguanosine triphosphate (8-oxo-dGTP), a mutagenic substrate for DNA synthesis, in the nucleotide pool, thereby preventing DNA replication errors. During a search of GenBank EST database, we found a new member of MutT-related protein, MTH2, which possesses the 23-amino acid MutT module. The cloned mouse MTH2 (mMTH2) cDNA was expressed in Escherichia coli mutT(-) cells and the protein was purified. mMTH2 protein hydrolyzes 8-oxo-dGTP to 8-oxo-dGMP, with Km of 32 microM. Expression of cDNA for mMTH2 reduced significantly the elevated level of spontaneous mutation frequency of E. coli mutT(-) cells. Thus, MTH2 has a potential to protect the genetic material from the untoward effects of endogenous oxygen radicals. MTH2 could act as an MTH1 redundancy factor. |
