First Author | Reid LL | Year | 1997 |
Journal | Biochim Biophys Acta | Volume | 1352 |
Issue | 3 | Pages | 233-7 |
PubMed ID | 9224945 | Mgi Jnum | J:41485 |
Mgi Id | MGI:893963 | Doi | 10.1016/s0167-4781(97)00058-4 |
Citation | Reid LL, et al. (1997) Molecular cloning and sequencing of the cDNA encoding the catalytic subunit of mouse glutamate-cysteine ligase. Biochim Biophys Acta 1352(3):233-7 |
abstractText | Reverse transcription-polymerase chain reaction (RT-PCR) was used for the enzymatic synthesis of cDNA sequences encompassing the open reading frame for the catalytic subunit of mouse kidney glutamate-cysteine ligase (Glclc). Comparison of the mouse Glclc cDNA sequence and predicted protein sequence with that of rat Glclc and human GLCLC revealed between 94.8% and 88.4% cDNA homology and 98.4% to 95% amino acid identity, respectively. |