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Publication : Molecular cloning and sequencing of the cDNA encoding the catalytic subunit of mouse glutamate-cysteine ligase.

First Author  Reid LL Year  1997
Journal  Biochim Biophys Acta Volume  1352
Issue  3 Pages  233-7
PubMed ID  9224945 Mgi Jnum  J:41485
Mgi Id  MGI:893963 Doi  10.1016/s0167-4781(97)00058-4
Citation  Reid LL, et al. (1997) Molecular cloning and sequencing of the cDNA encoding the catalytic subunit of mouse glutamate-cysteine ligase. Biochim Biophys Acta 1352(3):233-7
abstractText  Reverse transcription-polymerase chain reaction (RT-PCR) was used for the enzymatic synthesis of cDNA sequences encompassing the open reading frame for the catalytic subunit of mouse kidney glutamate-cysteine ligase (Glclc). Comparison of the mouse Glclc cDNA sequence and predicted protein sequence with that of rat Glclc and human GLCLC revealed between 94.8% and 88.4% cDNA homology and 98.4% to 95% amino acid identity, respectively.
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