| First Author | Hägele S | Year | 2006 |
| Journal | FEBS Lett | Volume | 580 |
| Issue | 15 | Pages | 3731-8 |
| PubMed ID | 16777103 | Mgi Jnum | J:110340 |
| Mgi Id | MGI:3640039 | Doi | 10.1016/j.febslet.2006.05.058 |
| Citation | Hagele S, et al. (2006) TSGA10 prevents nuclear localization of the hypoxia-inducible factor (HIF)-1alpha. FEBS Lett 580(15):3731-8 |
| abstractText | The hypoxia-inducible factor (HIF)-1 is a transcriptional regulator of genes involved in oxygen homeostasis. We previously described testis-specific isoforms of HIF-1alpha (mHIF-1alphaI.1 and hHIF-1alphaTe). Using mHIF-1alpha exon I.1 knock-out mice we confirmed the specific expression of mHIF-1alphaI.1 in the sperm tail. A protein-protein interaction between HIF-1alpha and the testis specific gene antigen 10 (TSGA10) was identified by yeast two-hybrid screening. TSGA10 is expressed in testis but also in other organs and malignant tissues. Immunofluorescence analysis indicated that the C-terminal part of TSGA10 accumulates in the midpiece of spermatozoa, where it co-localizes with HIF-1alpha. HIF-1alpha nuclear localization and HIF-1 transcriptional activity were significantly affected by overexpressed TSGA10. |
