| First Author | Zhang W | Year | 1998 |
| Journal | Cell | Volume | 92 |
| Issue | 1 | Pages | 83-92 |
| PubMed ID | 9489702 | Mgi Jnum | J:54183 |
| Mgi Id | MGI:1334760 | Doi | 10.1016/s0092-8674(00)80901-0 |
| Citation | Zhang W, et al. (1998) LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation. Cell 92(1):83-92 |
| abstractText | Despite extensive study, several of the major components involved in T cell receptor-mediated signaling remain unidentified. Here we report the cloning of the cDNA for a highly tyrosine-phosphorylated 36-38 kDa protein, previously characterized by its association with Grb2, phospholipase C-gamma1, and the p85 subunit of phosphoinositide 3- kinase. Deduced amino acid sequence identifies a novel integral membrane protein containing multiple potential tyrosine phosphorylation sites. We show that this protein is phosphorylated by ZAP-70/Syk protein tyrosine kinases leading to recruitment of multiple signaling molecules. Its function is demonstrated by inhibition of T cell activation following overexpression of a mutant form lacking critical tyrosine residues. Therefore, we propose to name the molecule LAT- linker for activation of T cells. |
