| First Author | Liljedahl M | Year | 2001 |
| Journal | Cell | Volume | 104 |
| Issue | 3 | Pages | 409-20 |
| PubMed ID | 11239398 | Mgi Jnum | J:180493 |
| Mgi Id | MGI:5306327 | Doi | 10.1016/s0092-8674(01)00228-8 |
| Citation | Liljedahl M, et al. (2001) Protein kinase D regulates the fission of cell surface destined transport carriers from the trans-Golgi network. Cell 104(3):409-20 |
| abstractText | When a kinase inactive form of Protein Kinase D (PKD-K618N) was expressed in HeLa cells, it localized to the trans-Golgi network (TGN) and caused extensive tubulation. Cargo that was destined for the plasma membrane was found in PKD-K618N-containing tubes but the tubes did not detach from the TGN. As a result, the transfer of cargo from TGN to the plasma membrane was inhibited. We have also demonstrated the formation and subsequent detachment of cargo-containing tubes from the TGN in cells stably expressing low levels of PKD-K618N. Our results suggest that PKD regulates the fission from the TGN of transport carriers that are en route to the cell surface. |
