| First Author | Terashima M | Year | 1991 |
| Journal | Arch Biochem Biophys | Volume | 285 |
| Issue | 2 | Pages | 231-7 |
| PubMed ID | 1897929 | Mgi Jnum | J:11446 |
| Mgi Id | MGI:59881 | Doi | 10.1016/0003-9861(91)90354-l |
| Citation | Terashima M, et al. (1991) Isolation of a full-length cDNA encoding mouse aromatase P450. Arch Biochem Biophys 285(2):231-7 |
| abstractText | A full-length cDNA clone for aromatase P450 has been isolated from a pregnant mouse ovarian cDNA library. The insert of this clone (2394 bp) contains a 1509-bp open reading frame encoding 503 amino acid residues together with a 46-bp 5'-untranslated stretch and an 839-bp 3'-untranslated region to which a poly(A) tract is attached. Northern blot analysis of ovarian RNA from pregnant mice reveals a major mRNA band of 2.5 kb with a minor band of 2.1 kb. Comparison of mouse aromatase P450 with that of rat, human, and chicken shows 91, 81, and 69% identity in the nucleotide sequence and 92, 79, and 69% identity in the deduced amino acid sequence, respectively. The membrane-spanning domain of mouse aromatase P450 is estimated to be an extremely hydrophobic segment located within the N-terminal region of the molecule. Furthermore, a highly conserved heme-binding domain is noticed. |
