| First Author | Abed M | Year | 2019 |
| Journal | PLoS One | Volume | 14 |
| Issue | 4 | Pages | e0214110 |
| PubMed ID | 30951545 | Mgi Jnum | J:273456 |
| Mgi Id | MGI:6293949 | Doi | 10.1371/journal.pone.0214110 |
| Citation | Abed M, et al. (2019) The Gag protein PEG10 binds to RNA and regulates trophoblast stem cell lineage specification. PLoS One 14(4):e0214110 |
| abstractText | Peg10 (paternally expressed gene 10) is an imprinted gene that is essential for placental development. It is thought to derive from a Ty3-gyspy LTR (long terminal repeat) retrotransposon and retains Gag and Pol-like domains. Here we show that the Gag domain of PEG10 can promote vesicle budding similar to the HIV p24 Gag protein. Expressed in a subset of mouse endocrine organs in addition to the placenta, PEG10 was identified as a substrate of the deubiquitinating enzyme USP9X. Consistent with PEG10 having a critical role in placental development, PEG10-deficient trophoblast stem cells (TSCs) exhibited impaired differentiation into placental lineages. PEG10 expressed in wild-type, differentiating TSCs was bound to many cellular RNAs including Hbegf (Heparin-binding EGF-like growth factor), which is known to play an important role in placentation. Expression of Hbegf was reduced in PEG10-deficient TSCs suggesting that PEG10 might bind to and stabilize RNAs that are critical for normal placental development. |
