| First Author | Nonaka S | Year | 1998 |
| Journal | Cell | Volume | 95 |
| Issue | 6 | Pages | 829-37 |
| PubMed ID | 9865700 | Mgi Jnum | J:51419 |
| Mgi Id | MGI:1316600 | Doi | 10.1016/s0092-8674(00)81705-5 |
| Citation | Nonaka S, et al. (1998) Randomization of left-right asymmetry due to loss of nodal cilia generating leftward flow of extraembryonic fluid in mice lacking KIF3B motor protein. Cell 95(6):829-37 |
| abstractText | Microtubule-dependent motor, murine KIF3B, was disrupted by gene targeting. The null mutants did not survive beyond midgestation, exhibiting growth retardation, pericardial sac ballooning, and neural tube disorganization. Prominently, the left-right asymmetry was randomized in the heart loop and the direction of embryonic turning. lefty-2 expression was either bilateral or absent. Furthermore, the node lacked monocilia while the basal bodies were present. Immunocytochemistry revealed KIF3B localization in wild-type nodal cilia. Video microscopy showed that these cilia were motile and generated a leftward flow. These data suggest that KIF3B is essential for the left-right determination through intraciliary transportation of materials for ciliogenesis of motile primary cilia that could produce a gradient of putative morphogen along the left-right axis in the node. |
