| First Author | Dawson SJ | Year | 1996 |
| Journal | J Biol Chem | Volume | 271 |
| Issue | 20 | Pages | 11631-3 |
| PubMed ID | 8662774 | Mgi Jnum | J:38139 |
| Mgi Id | MGI:85526 | Doi | 10.1074/jbc.271.20.11631 |
| Citation | Dawson SJ, et al. (1996) A single amino acid change converts an inhibitory transcription factor into an activator. J Biol Chem 271(20):11631-3 |
| abstractText | The closely related POU family transcription factors Brn-3a and Brn-3b differ in their functional activity with Brn-3a activating several target promoters, which are repressed by Brn-3b. Brn-3b also prevents promoter activation by Brn-3a. Here we have altered a single isoleucine residue in the POU homeodomain of Brn-3b to the valine residue found at the equivalent position in Brn-3a. This change not only abolishes the ability of Brn-3b to repress basal and Brn-3a-stimulated promoter activity but also converts it to an activator of similar potency to Brn-3a. Hence a single amino acid difference determines the difference between an activator and a repressor in the Brn-3 family. |
