| First Author | Ouellette AJ | Year | 1992 |
| Journal | FEBS Lett | Volume | 304 |
| Issue | 2-3 | Pages | 146-8 |
| PubMed ID | 1618314 | Mgi Jnum | J:1458 |
| Mgi Id | MGI:49985 | Doi | 10.1016/0014-5793(92)80606-h |
| Citation | Ouellette AJ, et al. (1992) Purification and primary structure of murine cryptdin-1, a Paneth cell defensin. FEBS Lett 304(2-3):146-8 |
| abstractText | We have purified and determined the amino acid sequence of cryptdin-1, a murine Paneth cell defensin. The peptide corresponds to a previously characterized mRNA that accumulates to high abundance during postnatal ontogeny of the small bowel. Acid-extracted intestinal protein was fractionated by cation-exchange chromatography and fractions were assayed for antimicrobial activity. One peak of anti-Salmonella activity contained a putative defensin, based on its predicted electrophoretic migration in acid-urea PAGE. The peptide was purified to homogeneity by RP-HPLC and sequenced. These studies demonstrate defensin expression in non-myeloid tissue. The N-terminal extension of cryptdin-1 is a unique structural feature of this novel epithelial defensin. |
