| First Author | Noh MJ | Year | 1995 |
| Journal | Biochem Mol Biol Int | Volume | 35 |
| Issue | 6 | Pages | 1205-13 |
| PubMed ID | 7492958 | Mgi Jnum | J:31058 |
| Mgi Id | MGI:78519 | Citation | Noh MJ, et al. (1995) Sequences responsible for transcription termination of the mouse gastrin gene. Biochem Mol Biol Int 35(6):1205-13 |
| abstractText | To identify the transcription termination elements in the mouse gastrin gene, we examined RNA transcripts after stable transfection of gastrin expression plasmids into the NIH3T3 cell line. The GT-repeat region at the 3'-flanking sequence of the mouse gastrin gene acted as a transcription terminator. When the GT-repeat unit was deleted from its site, the effect of termination disappeared. Further experiment, using serial deletion mutants, revealed that the 56-38 nucleotide upstream region from the GT-repeat unit also participated in transcription termination. We propose that the upstream region of the GT-repeat unit might be recognized as a pause site by the RNA polymerase II, and an abnormal DNA structure, derived from the GT-repeat unit, might function as a blockage of polymerase processivity. |
