| First Author | Tetzlaff MT | Year | 2004 |
| Journal | Mol Cell Biol | Volume | 24 |
| Issue | 6 | Pages | 2487-98 |
| PubMed ID | 14993286 | Mgi Jnum | J:89060 |
| Mgi Id | MGI:3038028 | Doi | 10.1128/MCB.24.6.2487-2498.2004 |
| Citation | Tetzlaff MT, et al. (2004) Cyclin F disruption compromises placental development and affects normal cell cycle execution. Mol Cell Biol 24(6):2487-98 |
| abstractText | Human cyclin F was originally isolated as a cDNA capable of suppressing the temperature sensitivity of a Saccharomyces cerevisiae cdc4-1 mutant. Its tightly regulated expression and high conservation in the evolutionary progression from amphibians to mammals suggest that it coordinates the timing of a critical cell cycle event. The fact that it contains an F box and can form an SCF (Skp1-Cul1/Cdc53-F-box) complex in vivo further suggests that it may also function in proteolysis. To investigate the role of cyclin F in vivo, we generated mice deficient for cyclin F and conditionally deficient mice as well as mouse embryonic fibroblasts (MEFs) conditionally deficient for cyclin F. Heterozygous animals are normal and fertile, but CycF(-/-) animals, with a myriad of developmental anomalies due in large part to failures in yolk sac and chorioallantoic placentation, die around embryonic day 10.5. Tissue-specific deletion of cyclin F revealed that it was not required for the development and function of a number of different embryonic and adult tissues. In contrast, MEFs lacking cyclin F, while viable, do exhibit cell cycle defects, including reduced population-doubling time and a delay in cell cycle reentry from quiescence, indicating that cyclin F plays a role in cell cycle regulation. |
