| First Author | Gil S | Year | 2006 |
| Journal | Dev Biol | Volume | 298 |
| Issue | 1 | Pages | 201-11 |
| PubMed ID | 16925989 | Mgi Jnum | J:119572 |
| Mgi Id | MGI:3702801 | Doi | 10.1016/j.ydbio.2006.06.029 |
| Citation | Gil S, et al. (2006) The enigma of ATCE1, an acrosome-associated transcription factor. Dev Biol 298(1):201-11 |
| abstractText | Atce1 belongs to the CREB3/LZIP subtype of the ATF/CREB transcription factor gene family. Its transcription has previously been shown to be testis-specific and within the testis to be restricted to haploid spermatids. In this study, we characterized the protein's distribution in the testis and found that it accumulates in late round and in elongating spermatids, corresponding to developmental stages considered transcriptionally silent. ATCE1 accumulation is acrosome-specific and persists up to mature epididymal cells, at which stage the protein remained associated with the inner acrosome membrane even after acrosomal reaction. No nuclear localization was evident at any spermatogenic stage. Expression of full-length ATCE1 in various cell lines revealed ER and Golgi localization whereas truncation of the C-terminus allowed entrance into the nucleus. Potent transcriptional activation activity, from kB-containing regulatory elements (but not from CRE elements as one might expect), was observed using the C-terminally truncated nuclear form of ATCE1. These results raise the question of why would a transcription factor be specifically anchored to the acrosome inner membrane? An intriguing speculation that ATCE1 might be paternally delivered to the newly formed zygote is discussed. |
