| First Author | Fujita H | Year | 1998 |
| Journal | J Biol Chem | Volume | 273 |
| Issue | 41 | Pages | 26516-21 |
| PubMed ID | 9756887 | Mgi Jnum | J:143699 |
| Mgi Id | MGI:3828419 | Doi | 10.1074/jbc.273.41.26516 |
| Citation | Fujita H, et al. (1998) Interaction of Hic-5, A senescence-related protein, with focal adhesion kinase. J Biol Chem 273(41):26516-21 |
| abstractText | Hydrogen peroxide-inducible clone (Hic)-5 is induced during the senescent process in human fibroblasts, and the overexpression of Hic-5 induces a senescence-like phenotype. Structurally, Hic-5 and paxillin, a 68-kDa cytoskeletal protein, share homology such as the LD motifs in the N-terminal half and the LIM domains in the C-terminal half. Here we show that Hic-5 binds to focal adhesion kinase (FAK) by its N-terminal domain, and is localized to focal adhesions by its C-terminal LIM domains. However, Hic-5 is not tyrosine phosphorylated either by the coexpressed FAK in COS cells or by integrin stimulation in 293T cells. Furthermore, overexpression of Hic-5 results in a decreased tyrosine phosphorylation of paxillin. These findings suggest that putative functions of Hic-5 are the recruitment of FAK to focal adhesions and a competitive inhibition of tyrosine phosphorylation of paxillin. |
