| First Author | Moore SK | Year | 1987 |
| Journal | Gene | Volume | 56 |
| Issue | 1 | Pages | 29-40 |
| PubMed ID | 2445630 | Mgi Jnum | J:8924 |
| Mgi Id | MGI:57389 | Doi | 10.1016/0378-1119(87)90155-7 |
| Citation | Moore SK, et al. (1987) Cloning and nucleotide sequence of the murine hsp84 cDNA and chromosome assignment of related sequences. Gene 56(1):29-40 |
| abstractText | The nucleotide (nt) sequence of mouse 84-kDa heat shock protein (Hsp) cDNA has been determined using a combination of molecular cloning and oligodeoxynucleotide priming on poly(A) + RNA. The cDNA was 2.5 kb long, not including the poly(A) tail. It contained a 5' leader of about 94 nt that was G + C-rich, and a 243-nt 3'-untranslated region that was A + T-rich in the vicinity of the polyadenylation signal. Gene hsp84 codes for an acidic polypeptide of 724 amino acid (aa) residues. Mouse Hsp84 had 81% and 63% aa homology to Drosophila melanogaster Hsp82 and yeast Hsp90, respectively. The nucleotide sequence had 74% and 59% homology to Drosophila and yeast hsp sequences, respectively, in the coding regions of these genes. This homology did not extend to the 5' - and 3'-untranslated regions. Chromosomal analysis indicated that hsp84-related sequences are on at least three different chromosomes. |
