| First Author | Tsuji T | Year | 2011 |
| Journal | Cell Mol Neurobiol | Volume | 31 |
| Issue | 5 | Pages | 663-8 |
| PubMed ID | 21350944 | Mgi Jnum | J:177235 |
| Mgi Id | MGI:5294522 | Doi | 10.1007/s10571-011-9668-3 |
| Citation | Tsuji T, et al. (2011) Ect2, an ortholog of Drosophila's pebble, negatively regulates neurite outgrowth in neuroblastoma x glioma hybrid NG108-15 cells. Cell Mol Neurobiol 31(5):663-8 |
| abstractText | To identify genes required for brain development, we previously performed in vivo RNA interference (RNAi) screening in Drosophila embryos. We identified pebble as a gene that disrupts development of the Drosophila nervous system. Although pebble has been shown to be involved in neuronal development of Drosophila in several screens, the involvement of Ect2, a mammalian ortholog of pebble, in mammalian neuronal development has not been addressed. To examine the role of Ect2 in neuronal differentiation, we performed Ect2 RNAi in the mouse neuroblastoma x rat glioma NG108-15 cell line. Depletion of Ect2 resulted in an increased proportion of binucleate cells and morphological differentiation of NG108-15 cells characterized by the outgrowth of neurites. These morphological changes were correlated with an increased level of acetylcholine esterase mRNA. In addition, expression of Ect2 was decreased in differentiated NG108-15 cells induced by dibutyryl cyclic AMP. These findings indicate that Ect2 negatively regulates the differentiation of NG108-15 cells and suggest that Ect2 may play a role in neuronal differentiation and brain development in vivo. |
