| First Author | Birkenfeld J | Year | 2003 |
| Journal | Biochem Biophys Res Commun | Volume | 302 |
| Issue | 3 | Pages | 526-33 |
| PubMed ID | 12615066 | Mgi Jnum | J:82556 |
| Mgi Id | MGI:2653664 | Doi | 10.1016/s0006-291x(03)00182-7 |
| Citation | Birkenfeld J, et al. (2003) Characterization of zetin 1/rBSPRY, a novel binding partner of 14-3-3 proteins. Biochem Biophys Res Commun 302(3):526-33 |
| abstractText | 14-3-3 proteins are ubiquitously expressed proteins which serve as central adaptors in different signal transduction cascades. In this study, yeast two-hybrid screening of a rat brain cDNA library identified a novel gene product termed zetin 1/rBSPRY that interacts with 14-3-3zeta. The zetin 1/rBSPRY gene is ubiquitously expressed in a variety of rat tissues, with highest expression being found in testis. In adult brain, high levels of zetin 1/rBSPRY mRNA were observed in the hippocampus, cereberal cortex, and piriform cortex. Biochemical studies confirmed zetin 1/rBSPRY to interact with 14-3-3zeta. Transient co-transfection in COS 7 cells caused a partial redistribution of zetin 1/rBSPRY into 14-3-3zeta enriched submembranous foci at leading edges. Our results suggest a role for zetin 1/rBSPRY-14-3-3 interactions at specialized submembrane domains. |
