| First Author | Shin M | Year | 2020 |
| Journal | Cell Chem Biol | Volume | 27 |
| Issue | 3 | Pages | 314-321.e5 |
| PubMed ID | 31991095 | Mgi Jnum | J:287132 |
| Mgi Id | MGI:6415524 | Doi | 10.1016/j.chembiol.2020.01.005 |
| Citation | Shin M, et al. (2020) DAGL-Beta Functions as a PUFA-Specific Triacylglycerol Lipase in Macrophages. Cell Chem Biol 27(3):314-321.e5 |
| abstractText | Here, we apply quantitative chemical proteomics and untargeted lipidomics to assign a polyunsaturated fatty acid (PUFA)-specific triacylglycerol (TAG) lipase activity for diacylglycerol lipase-beta (DAGLbeta) in macrophages. We demonstrate that DAGLbeta but not DAGLalpha is expressed and active in bone marrow-derived macrophages (BMDMs) as determined by activity-based protein profiling analysis of SILAC BMDMs. Genetic disruption of DAGLbeta resulted in accumulation of cellular TAGs composed of PUFA but not saturated/low unsaturated fatty acid counterparts, which is recapitulated in wild-type macrophages treated with a DAGLbeta-selective inhibitor. Biochemical assays with synthetic substrates confirm PUFA-TAGs as authentic DAGLbeta substrates. In summary, our findings identify DAGLbeta as a PUFA-specific TAG lipase in primary macrophages. |
