| First Author | LeClair KP | Year | 1986 |
| Journal | EMBO J | Volume | 5 |
| Issue | 12 | Pages | 3227-34 |
| PubMed ID | 3028776 | Mgi Jnum | J:8594 |
| Mgi Id | MGI:57060 | Doi | 10.1002/j.1460-2075.1986.tb04633.x |
| Citation | LeClair KP, et al. (1986) Isolation of a murine Ly-6 cDNA reveals a new multigene family. EMBO J 5(12):3227-34 |
| abstractText | The Ly-6 alloantigens have been shown to play a critical role in T lymphocyte activation. To isolate a Ly-6 cDNA, synthetic oligonucleotides, based on the partial amino acid sequence of purified Ly-6E.1 protein, were used to probe a cDNA library. The synthetic oligonucleotides or the isolated cDNA detected a 1.1-kb RNA species. Sequence analysis of the cDNA clone revealed that the Ly-6E.1 protein consists of a 26-amino acid leader followed by a 108-residue, cysteine-rich, core protein with no N-linked glycosylation sites. Southern blot analysis of genomic DNAs revealed multiple bands indicating a family of related genes. Using recombinant inbred and Ly-6 congenic strains of mice, restriction fragment length polymorphisms were demonstrable, and correlated with the Ly-6 allotype of the DNA donors. This probe will enable further molecular genetic analysis of the role of Ly-6-linked proteins in the process of T lymphocyte activation. Isolation of Ly-6 genomic clones may promote a further understanding of the complex tissue-specific expression patterns characteristic of Ly-6-linked genes. |
