| First Author | Krieg P | Year | 1999 |
| Journal | FEBS Lett | Volume | 446 |
| Issue | 1 | Pages | 142-8 |
| PubMed ID | 10100631 | Mgi Jnum | J:46510 |
| Mgi Id | MGI:1313249 | Doi | 10.1016/s0014-5793(99)00196-9 |
| Citation | Krieg P, et al. (1999) Murine 12(R)-lipoxygenase: functional expression, genomic structure and chromosomal localization. FEBS Lett 446(1):142-8 |
| abstractText | A cDNA, recently cloned (by Krieg et al, (1998)) from mouse skin, was shown to encode a 12(R)-lipoxygenase. When expressed in HEK cells, the recombinant protein converted methyl arachidonate into the corresponding 12-HETE ester which was shown to be the R-enantiomer by chiral phase chromatography. Neither arachidonic acid nor linoleic acid sere substrates for the recombinant protein. The structure of the 12(R)-lipoxygenase gene is unique among all animal lipoxygenases in that it is divided into 15 exons and 14 introns spanning approximately 12.5 kh. By interspecific backcross analysis, the 12(R)-lipoxygenase gene was localized to the central region of mouse chromosome 11, (C) 1999 Federation of European Biochemical Societies. |
