| First Author | van Dijk KW | Year | 1995 |
| Journal | Biochim Biophys Acta | Volume | 1259 |
| Issue | 1 | Pages | 4-8 |
| PubMed ID | 7492614 | Mgi Jnum | J:29789 |
| Mgi Id | MGI:77307 | Doi | 10.1016/0005-2760(95)00158-9 |
| Citation | van Dijk KW, et al. (1995) Genomic and cDNA cloning of a novel mouse lipoxygenase gene. Biochim Biophys Acta 1259(1):4-8 |
| abstractText | A novel 12- and 15-lipoxygenase related gene was isolated from a mouse strain 129 genomic phage library in a screen with a human 15-lipoxygenase cDNA probe. The complete genomic sequence revealed 14 exons and 13 introns covering 7.3 kb of DNA. The splice junctions were verified from the cDNA sequences of a series of overlapping RT-PCR products. This novel mouse lipoxygenase gene was found to contain an open reading frame encoding 662 amino acids. The cDNA sequence is 68% identical to the mouse leukocyte type 12-lipoxygenase and 65% identical to the mouse platelet type 12-lipoxygenase cDNA. Nucleotide identities to the human 15-lipoxygenase cDNA and the human platelet type 12-lipoxygenase cDNA are 70% and 67%, respectively. The deduced amino acid sequence is 60% identical to the mouse platelet and leukocyte type 12-lipoxygenase sequences and the human 15- and 12-lipoxygenase sequences. This novel mouse lipoxygenase gene was found to be transcribed predominantly in epidermal tissue and named Aloxe. |
